Safranin O indicator is manufactured by Securex Lab Sciences. It is widely used in microscopy. It stains chromosomes as part of the Fleming triple stain. Gram staining is another protocol in which Safranin O indicator is used. Here, it is used as a counter stain for gram negative bacteria. Plant cell staining is done using Safranin-fast green FCF stain. It is a cationic dye. It stains the acidic proteoglycans in cartilage tissues. This is used in the medical field. Bound Safranin O produces an orange colour. It is used in histochemical analysis of mast cells. It is used for the detection of mucin too. Molecular formula: C20H19ClN4 Molecular mass: 350.54 g/mol Appearance: Crystal green