RDP TrioTM Reagent is supplied by HiMedia. HiMedia’s RDP TrioTM Reagent is designed for rapid purification of RNA, DNA and Protein from different samples. This product which is a mixture of guanidine thiocyanate and phenol in a mono-phase solution effectively dissolves RNA. After adding chloroform and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing protein. 1 ml of RDP TrioTM Reagent is sufficient to isolate RNA, DNA and Protein from 50-100 mg of tissue, 510X106 cells or 10 cm2 of culture dish surface, for cells grown in monolayer. This advanced procedure is an improvement to the single-step RNA isolation using phenol and guanidine isothiocyanate developed by Chomczynski and Sacchi. This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue and cells. It is advisable to store this product at room temperature (15-25°C).

HiMedia’s RDP TrioTM Reagent is for laboratory use only, not for drug, household or other uses. This product which is a mixture of guanidine thiocyanate and phenol in a mono-phase solution effectively dissolves RNA. Take appropriate laboratory safety measures and wear gloves when handling. Avoid contact with skin, and use eye protection. In case of contact, wash with large amount of water. Ribonucleases (RNases) are very stable and active enzymes that generally do not require cofactors to function. Since RNases are difficult to inactivate and even trace amounts are sufficient to destroy RNA, do not use any plasticware or glassware without first eliminating possible RNase contamination.