DNase I Solution (1mg/ml) is supplied by HiMedia. It is available in the form of a colourless solution. DNase I Solution (1mg/ml) consists of RNase free DNase I in a buffer which contains Sodium chloride, Magnesium chloride and Glycerol. This solution is supplied as ready to use.  DNase I is a glycosylated polypeptide which is commonly used for the degradation of single- and doublestranded DNAs (which are not required) into 5´ phosphodinucleotide and oligonucleotide fragments. The activity of DNase I is dependent on the presence of bivalent metal ions e.g. in the presence of Mg2+, Ca2+ or Zn2+ it degrades DNA by producing random single-strand nicks in the phosphate backbone and in the presence of Mn2+, both strands of double-stranded DNA are cleaved leaving blunt-end fragments. Monovalent metal ions like Na+ and K+ has a negative effect on DNase I.  It is advisable to store this product at – 20°C.

Deoxyribonuclease I is an RNase-free glycoprotein which specifically degrades DNA. It is a recombinant double strand specific endonuclease that catalyzes the degradation of single and double-stranded DNAs which result into di-, tri-, and oligonucleotides with 5′-phosphate and 3′-hydroxyl terminal. DNase I Solution is used for specific applications where integrity of RNA has to be maintained, e.g. for the removal of genomic DNA from RNA preparations prior to RT-PCR, during nick translations, for the isolation of DNAfree RNA after in vitro transcription reactions, DNase etc. Moreover, it is used to catalyze random degradation of single and double-stranded DNA. DNaseI solution is also used for the prevention of clumping of concentrated or cryopreserved hematopoietic cell suspensions following thawing.